⚗ Aliquot
Ligation ratio
Find the mass of insert to add for a target insert to vector molar ratio.
Insert mass for molar ratio
| m_{i} | Insert mass to add | ng |
| R | Insert to vector molar ratio | dimensionless |
| m_{v} | Vector mass | ng |
| L_{i} | Insert length | bp |
| L_{v} | Vector length | bp |
3:1, 1 kb insert into 5 kb vector
Use 30 ng of insert with 50 ng of vector (3:1).
docs/v3