⚗ Aliquot

Protein by A280

Calculate protein concentration from A280, its 0.1% extinction coefficient, and the path length.

Concentration from A280

c = \frac{A _{280}}{ε _{0.1%} \cdot ℓ}

c	Protein concentration	mg/mL
A_{280}	Absorbance at 280 nm	dimensionless
\varepsilon_{0.1\%}	0.1% extinction coefficient (A280 of 1 mg/mL, 1 cm)	mL/(mg cm)
\ell	Path length	cm

BSA, A280 0.667

Your protein is 1 mg/mL.

docs/v3