Work out the protein concentration. Every answer here is computed by the same tested calculator the rest of the site uses, so a solution can never drift away from the maths.
Work out the protein concentration.
Your protein is 0.1499 mg/mL.
conc = A280 / (E0.1% · path)conc = 0.1 / (0.667 · 1 cm) = 0.1499 mg/mLOpen this in the calculator to change the numbers.
Work out the protein concentration.
Your protein is 0.2249 mg/mL.
conc = A280 / (E0.1% · path)conc = 0.15 / (0.667 · 1 cm) = 0.2249 mg/mLOpen this in the calculator to change the numbers.
Work out the protein concentration.
Your protein is 0.3748 mg/mL.
conc = A280 / (E0.1% · path)conc = 0.25 / (0.667 · 1 cm) = 0.3748 mg/mLOpen this in the calculator to change the numbers.
Work out the protein concentration.
Your protein is 1.124 mg/mL.
conc = A280 / (E0.1% · path)conc = 0.75 / (0.667 · 1 cm) = 1.124 mg/mLOpen this in the calculator to change the numbers.
Work out the protein concentration.
Your protein is 0.4498 mg/mL.
conc = A280 / (E0.1% · path)conc = 0.3 / (0.667 · 1 cm) = 0.4498 mg/mLOpen this in the calculator to change the numbers.
Work out the protein concentration.
Your protein is 0.2999 mg/mL.
conc = A280 / (E0.1% · path)conc = 0.2 / (0.667 · 1 cm) = 0.2999 mg/mLOpen this in the calculator to change the numbers.
Work out the protein concentration.
Your protein is 0.7496 mg/mL.
conc = A280 / (E0.1% · path)conc = 0.5 / (0.667 · 1 cm) = 0.7496 mg/mLOpen this in the calculator to change the numbers.
Work out the protein concentration.
Your protein is 0.5997 mg/mL.
conc = A280 / (E0.1% · path)conc = 0.4 / (0.667 · 1 cm) = 0.5997 mg/mLOpen this in the calculator to change the numbers.
More protein practice
Total units and specific activity from a measured rate.
Open it in the calculator and it shows the same working step by step, with the units checked and the hazards flagged. Free, no account, and it keeps working offline once the page has loaded.
Open the Protein by A280 calculator