⚗ Aliquot

Beer-Lambert (absorbance to concentration)

Concentration from a spectrophotometric reading, for any extinction-coefficient convention.

Beer-Lambert law

A = ε \cdot c \cdot ℓ

A	absorbance	dimensionless
ε	molar extinction coefficient	M⁻¹cm⁻¹
c	concentration	mol/L
ℓ	path length	cm

NADH, A 0.622 at 340 nm (ε 6220)

Your sample is 100 µM at 340 nm.

Aliquot docs/v4/30-beer-lambert.md

c = \frac{A \cdot D F}{ε \cdot ℓ}

c	concentration	mol/L
DF	dilution factor	dimensionless
ε	molar extinction coefficient	M⁻¹cm⁻¹

Aliquot docs/v4/30-beer-lambert.md

c_{(g / L)} = \frac{A \cdot D F \cdot 10}{E _{1%} \cdot ℓ}

E₁%	1% specific extinction	dimensionless
c	concentration	g/L

Aliquot docs/v4/30-beer-lambert.md

c_{(m g / m L)} = \frac{A \cdot D F}{E _{0.1%} \cdot ℓ}

E₀.₁%	0.1% specific extinction	dimensionless
c	concentration	mg/mL

Aliquot docs/v4/30-beer-lambert.md