Spectrophotometry
Beer-Lambert (absorbance to concentration)
Concentration from absorbance, any extinction-coefficient convention.
A = ε · c · l
A starting point. Editing any field below overrides the preset.
Molar in M⁻¹cm⁻¹ (e.g. NADH 6220 at 340 nm); E1% and E0.1% are unitless. No safe generic value.
Needed only to also report mass units when you entered a molar ε (or molar units when you entered a mass-based ε).
Protocol result
⚗ Aliquot
Beer-Lambert (absorbance to concentration) · c (mol/L) = A · DF / (ε · l) · the lab bench, calculated
Beer-Lambert✓ c (mol/L) = A · DF / (ε · l)
Your sample is 100 µM at 340 nm.
1
Concentration: 100 µM.
Wavelength
340 nm
Absorbance
0.622
Concentration
100 µM