⚗ Aliquot
PCR master mix
Scale a per-reaction PCR recipe up to a full batch with an overage cushion, then back-fill water.
Effective reactions
| n_{\text{eff}} | Effective reactions (samples plus overage) | dimensionless |
| n_{\text{samp}} | Number of samples | dimensionless |
| p | Overage percentage | % |
docs/v3
Component volume for mix
| V_i | Volume of component i for the whole mix | uL |
| v_i | Per-reaction volume of component i | uL |
| n_{\text{samp}} | Number of samples | dimensionless |
| p | Overage percentage | % |
docs/v3
Water back-fill per reaction
| v_{\text{H2O}} | Water volume per reaction | uL |
| V_{\text{rxn}} | Reaction volume | uL |
| v_i | Per-reaction volume of component i | uL |
docs/v3
Total mix volume
| V_{\text{tot}} | Total master mix volume | uL |
| V_{\text{rxn}} | Reaction volume | uL |
| n_{\text{eff}} | Effective reactions | dimensionless |
docs/v3