Molecular biology
PCR master mix
Scale a per-reaction recipe to a batch, with overage.
mix = per-reaction x samples x (1 + overage)
ComponentµL / rxn
Protocol result
⚗ Aliquot
PCR master mix · volume = perReactionUl · samples · (1 + overagePct / 100) · the lab bench, calculated
Master mix✓ volume = perReactionUl · samples · (1 + overagePct / 100)
Master mix for 26.4 reactions (24 samples plus overage), total 528 µL.
1
Combine the mix volumes below, vortex, then aliquot 20 µL into each tube.
| Component | µL / rxn | µL for mix |
|---|---|---|
| 2x master mix | 10 | 264 |
| Forward primer (10 µM) | 1 | 26.4 |
| Reverse primer (10 µM) | 1 | 26.4 |
| Template | 2 | 52.8 |
| Water | 6 | 158.4 |
Reactions
26.4
Per reaction
20 µL
Total mix
528 µL