Cell culture

OD600 / cell density

OD600 to cells per mL, and back-dilute a culture to a target OD.

cells/mL = OD x factor
Shown is E. coli (8e8). Change for your organism.
Protocol result
⚗ Aliquot
OD600 / cell density · cells/mL = OD600 · factor · the lab bench, calculated
Cell density✓ cells/mL = OD600 · factor
Calculated live

That culture is about 4.8 × 10⁸ cells/mL.

1

Density: 4.8 × 10⁸ cells/mL.

OD600
0.6
Cells/mL
4.8 × 10⁸
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How do you convert OD600 to cells per mL?
A worked example with every step shown, and where it usually goes wrong.
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Ten problems with worked solutions, every answer computed by this calculator.
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OD600 / cell density, Aliquot