Dilutions
Serial dilution
A fold-dilution series, tube by tube.
Cₙ = C₀ / fⁿ
Protocol result
⚗ Aliquot
Serial dilution · Cₙ = C_stock / fⁿ ; V_t = V₂ / f ; Diluent = V₂ − V_t · the lab bench, calculated
Serial dilution✓ Cₙ = C_stock / fⁿ ; V_t = V₂ / f ; Diluent = V₂ − V_t
4 tubes at a 10× dilution, from 1 M down to 0.0001 M.
1
Pre-fill each tube with 0.9 mL of diluent.
2
Transfer 0.1 mL from the stock into tube 1, mix, then carry the same volume forward each step.
| Tube | Concentration | Transfer | Diluent |
|---|---|---|---|
| 1 | 0.1 M | 0.1 mL | 0.9 mL |
| 2 | 0.01 M | 0.1 mL | 0.9 mL |
| 3 | 0.001 M | 0.1 mL | 0.9 mL |
| 4 | 0.0001 M | 0.1 mL | 0.9 mL |
Tubes
4
Fold
10×
Transfer
0.1 mL
Diluent
0.9 mL