Molecular biology

DNA: ng to pmol

Convert a dsDNA mass to moles (and back) from its length.

pmol = ng x 1000 / (660 x bp)
Protocol result
⚗ Aliquot
DNA: ng to pmol · pmol = mass_ng * 1000 / (660 * bp) · the lab bench, calculated
DNA molarity✓ pmol = mass_ng * 1000 / (660 * bp)
Calculated live

1000 bp dsDNA: 0.07576 pmol.

1

Result: 0.07576 pmol.

Length
1000 bp
Mass
50 ng
Result
0.07576 pmol
Ask Curie
Learn and practise
How do you calculate dNA: ng to pmol?
A worked example with every step shown, and where it usually goes wrong.
Practice problems
Ten problems with worked solutions, every answer computed by this calculator.
Related calculators
Oligo resuspension
Resuspend a lyophilized primer to a stock concentration.
Ligation ratio
Insert mass for a target insert:vector molar ratio.
Nucleic acid (A260)
DNA and RNA concentration and purity from absorbance.
Nucleic acid copy number
Mass, molarity, and copies/µL for dsDNA, ssDNA, or ssRNA, both directions.
Oligo melting temperature (Tm)
Nearest-neighbor Tm with Na+ and Mg2+ salt corrections. Not a Wallace guess.
Gibson / ligation planner
Vector amount and insert ratios to an exact pipetting table.
All 57 calculators
Browse the full catalog by category.
DNA: ng to pmol, Aliquot