Molecular biology

Nucleic acid (A260)

DNA and RNA concentration and purity from absorbance.

c = A260 x factor x dilution
Protocol result
⚗ Aliquot
Nucleic acid (A260) · conc = A260 · factor · dilution · the lab bench, calculated
A260 quant✓ conc = A260 · factor · dilution
Calculated live

Your sample is 25 µg/mL.

1

Concentration: 25 µg/mL.

ℹ

No A280 provided, the 260/280 purity ratio was not computed.

A260
0.5
Concentration
25 µg/mL
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How do you calculate DNA concentration from A260?
A worked example with every step shown, and where it usually goes wrong.
Practice problems
Ten problems with worked solutions, every answer computed by this calculator.
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Nucleic acid (A260), Aliquot