Molecular biology

Oligo melting temperature (Tm)

Nearest-neighbor Tm with Na+ and Mg2+ salt corrections. Not a Wallace guess.

Tm = ΔH / (ΔS + R·ln(C_T/x)), salt-corrected
A/C/G/T/U, up to 200 nt. Below 14 nt falls back to the Wallace rule (flagged).
Chelates Mg2+; subtracted in the monovalent-equivalent.
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Oligo melting temperature (Tm), Aliquot