Physical
HPLC method transfer
Scale flow, injection, and gradient between columns, with void volume and dead time.
F₂ = F₁(d₂/d₁)²(dp₁/dp₂)
Protocol result
⚗ Aliquot
HPLC method transfer · F₂ = F₁(d₂/d₁)²(dp₁/dp₂); Vi₂ = Vi₁(d₂²L₂)/(d₁²L₁) · the lab bench, calculated
HPLC transfer✓ F₂ = F₁(d₂/d₁)²(dp₁/dp₂); Vi₂ = Vi₁(d₂²L₂)/(d₁²L₁)
New method: 0.579 mL/min, 1.389 µL inj, 3.6 min gradient
1
Scaled to keep separation: flow by bore² and particle ratio, injection and gradient by column volume.
⚠
Geometric transfer preserves separation only if stationary-phase chemistry, temperature, and mobile phase are unchanged. Re-verify resolution after transfer.
New flow
0.579 mL/min
New injection
1.389 µL
New gradient
3.6 min
New t0
0.203 min