Physical
How do you calculate hPLC method transfer?
Scale flow, injection, and gradient between columns, with void volume and dead time. The relationship used is F₂ = F₁(d₂/d₁)²(dp₁/dp₂).
Worked example
Every number below is computed by the calculator, not typed in
What you are given
- Old i.d. (mm): 4.6
- Old length (mm): 150
- Old particle (µm): 5
- Old flow (mL/min): 1.0
- Old injection (µL): 20
- Old gradient (min, optional): 30
- New i.d. (mm): 2.1
- New length (mm): 50
- New particle (µm): 1.8
Formula: F₂ = F₁(d₂/d₁)²(dp₁/dp₂)
The working
- Flow:
F₁ (d₂/d₁)² (dp₁/dp₂) = 0.579 mL/min - Injection:
Vi₁ (d₂²L₂)/(d₁²L₁) = 1.389 µL - Gradient time:
tg₁ (F₁/F₂)(V₂/V₁) = 3.6 min
New method: 0.579 mL/min, 1.389 µL inj, 3.6 min gradient
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