Molecular biology
Nucleic acid (A260): practice problems Work out the concentration and the purity ratio. Every answer here is computed by the same tested calculator the rest of the site uses, so a solution can never drift away from the maths.
Problem 1 Nucleic acid (A260) Work out the concentration and the purity ratio.
A260: 0.5 Nucleic acid: dsDNA (x50) Dilution factor: 50 Show the answer and the working Your sample is 1250 µg/mL.
Apply OD factor: conc = 0.5 · 50 (dsDNA) · 50 = 1250 µg/mL Open this in the calculator to change the numbers.
Problem 2 Nucleic acid (A260) Work out the concentration and the purity ratio.
A260: 1 Nucleic acid: dsDNA (x50) Dilution factor: 50 Show the answer and the working Your sample is 2500 µg/mL.
Apply OD factor: conc = 1 · 50 (dsDNA) · 50 = 2500 µg/mL Open this in the calculator to change the numbers.
Problem 3 Nucleic acid (A260) Work out the concentration and the purity ratio.
A260: 4 Nucleic acid: dsDNA (x50) Dilution factor: 50 Show the answer and the working Your sample is 10000 µg/mL.
Apply OD factor: conc = 4 · 50 (dsDNA) · 50 = 10000 µg/mL Open this in the calculator to change the numbers.
Problem 4 Nucleic acid (A260) Work out the concentration and the purity ratio.
A260: 1.5 Nucleic acid: dsDNA (x50) Dilution factor: 50 Show the answer and the working Your sample is 3750 µg/mL.
Apply OD factor: conc = 1.5 · 50 (dsDNA) · 50 = 3750 µg/mL Open this in the calculator to change the numbers.
Problem 5 Nucleic acid (A260) Work out the concentration and the purity ratio.
A260: 3 Nucleic acid: dsDNA (x50) Dilution factor: 50 Show the answer and the working Your sample is 7500 µg/mL.
Apply OD factor: conc = 3 · 50 (dsDNA) · 50 = 7500 µg/mL Open this in the calculator to change the numbers.
Problem 6 Nucleic acid (A260) Work out the concentration and the purity ratio.
A260: 2 Nucleic acid: dsDNA (x50) Dilution factor: 50 Show the answer and the working Your sample is 5000 µg/mL.
Apply OD factor: conc = 2 · 50 (dsDNA) · 50 = 5000 µg/mL Open this in the calculator to change the numbers.
More molecular biology practice
Same topic, different calculation Resuspend a lyophilized primer to a stock concentration.
Convert a dsDNA mass to moles (and back) from its length.
Insert mass for a target insert:vector molar ratio.
Mass, molarity, and copies/µL for dsDNA, ssDNA, or ssRNA, both directions.
Stuck on one of them? Open it in the calculator and it shows the same working step by step, with the units checked and the hazards flagged. Free, no account, and it keeps working offline once the page has loaded.
Open the Nucleic acid (A260) calculator