Molecular biology
qPCR fold change (ddCt)
Livak 2^-ddCt with proper error propagation and an honest asymmetric band.
fold = 2^(−ΔΔCt); band = 2^(−(ΔΔCt ± SD))
Protocol result
⚗ Aliquot
qPCR fold change (ddCt) · fold = 2^(−ΔΔCt); band = 2^(−(ΔΔCt ± SD)) · the lab bench, calculated
qPCR ddCt✓ fold = 2^(−ΔΔCt); band = 2^(−(ΔΔCt ± SD))
Fold change 4x (up), 95% band 2.9 to 5.52.
1
ΔΔCt -2 ± 0.46.
ℹ
Livak method assumes ~100% amplification efficiency (E=2) for both target and reference. Use a standard curve / Pfaffl method if efficiencies differ.
Fold change
4x
Range
2.9 to 5.52
ΔΔCt
-2 ± 0.46