Molecular biology
How do you calculate gibson / ligation planner?
Vector amount and insert ratios to an exact pipetting table. The relationship used is V = (vector_pmol · ratio) / (stock_ng/µL · pmol_per_ng).
Worked example
Every number below is computed by the calculator, not typed in
What you are given
- Vector amount (pmol): 0.05
- Reaction volume (µL): 20
- Fragments: [{"name":"Vector","role":"vector","length":"5000","stock":"50","ratio":"1"},{"name":"Insert","role":"insert","length":"1000","stock":"25","ratio":"2"}]
Formula: V = (vector_pmol · ratio) / (stock_ng/µL · pmol_per_ng)
The working
- Vector:
target = 0.05 pmol - Per fragment:
pmol = vector · ratio; V = pmol / (ng/µL · 1000/(bp·660))
Pipette 2 fragments into 20 µL.
Open this example in the calculator
Do it with your own numbers.
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