What you are given
- Length: 1000
- Value: 1
Formula: copies/µL = (ng/µL · 1e-9 / MW) · N_A
Molecular biology
How do you calculate nucleic acid copy number?
Mass, molarity, and copies/µL for dsDNA, ssDNA, or ssRNA, both directions. The relationship used is copies/µL = (ng/µL · 1e-9 / MW) · N_A.
Worked example
Every number below is computed by the calculator, not typed in
The working
- MW:
1000 residues · 660 g/mol=660000 g/mol - Copies/µL:
conc · 6.022e14 / MW=9.124e+8
That is 9.124e+8 copies/µL.
Try it yourself
Two problems, with the working revealed
Problem 1 Nucleic acid copy number
Work out the result for nucleic acid copy number.
- Length: 2000
- Value: 1.5
Show the answer and the working
That is 6.843e+8 copies/µL.
- MW:
2000 residues · 660 g/mol=1320000 g/mol - Copies/µL:
conc · 6.022e14 / MW=6.843e+8
Open this in the calculator to change the numbers.
Problem 2 Nucleic acid copy number
Work out the result for nucleic acid copy number.
- Length: 3000
- Value: 4
Show the answer and the working
That is 1.217e+9 copies/µL.
- MW:
3000 residues · 660 g/mol=1980000 g/mol - Copies/µL:
conc · 6.022e14 / MW=1.217e+9
Open this in the calculator to change the numbers.
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