What you are given
- Nominal volume (µL): 100
- Water temperature (°C): 20
- Weighed masses (mg): 99.7, 99.8, 100.1, 99.9, 100.0, 99.8, 100.2, 99.9, 100.0, 99.7
Formula: V = mass × Z
Physical
How do you calculate pipette calibration (ISO 8655)?
Turn weighed water masses into accuracy and precision against the nominal volume. The relationship used is V = mass × Z.
Worked example
Every number below is computed by the calculator, not typed in
The working
- Z factor:
water density + buoyancy=1.003 µL/mg - Mean volume:
mean(mass) × Z=100.2 µL - Systematic error:
mean − nominal=0.2 µL (0.2%) - Random error:
SD, CV=0.167 µL (0.166%)
Mean 100.2 µL, accuracy +0.2%, CV 0.166%
Do it with your own numbers.
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