Work out the result for ligation ratio. Every answer here is computed by the same tested calculator the rest of the site uses, so a solution can never drift away from the maths.
Work out the result for ligation ratio.
Use 15 ng of insert with 20 ng of vector (3:1).
insertBp / vectorBp = 0.253 · 20 ng · (1000 / 4000) = 15 ngOpen this in the calculator to change the numbers.
Work out the result for ligation ratio.
Use 18.75 ng of insert with 15 ng of vector (2.5:1).
insertBp / vectorBp = 0.52.5 · 15 ng · (1000 / 2000) = 18.75 ngOpen this in the calculator to change the numbers.
Work out the result for ligation ratio.
Use 7 ng of insert with 10 ng of vector (3.5:1).
insertBp / vectorBp = 0.23.5 · 10 ng · (1000 / 5000) = 7 ngOpen this in the calculator to change the numbers.
Work out the result for ligation ratio.
Use 12 ng of insert with 20 ng of vector (3:1).
insertBp / vectorBp = 0.23 · 20 ng · (1000 / 5000) = 12 ngOpen this in the calculator to change the numbers.
Work out the result for ligation ratio.
Use 262.5 ng of insert with 75 ng of vector (3.5:1).
insertBp / vectorBp = 13.5 · 75 ng · (5000 / 5000) = 262.5 ngOpen this in the calculator to change the numbers.
Work out the result for ligation ratio.
Use 70 ng of insert with 20 ng of vector (3.5:1).
insertBp / vectorBp = 13.5 · 20 ng · (1000 / 1000) = 70 ngOpen this in the calculator to change the numbers.
Work out the result for ligation ratio.
Use 100 ng of insert with 25 ng of vector (5:1).
insertBp / vectorBp = 0.85 · 25 ng · (4000 / 5000) = 100 ngOpen this in the calculator to change the numbers.
Work out the result for ligation ratio.
Use 100 ng of insert with 75 ng of vector (1:1).
insertBp / vectorBp = 1.3331 · 75 ng · (4000 / 3000) = 100 ngOpen this in the calculator to change the numbers.
Work out the result for ligation ratio.
Use 40 ng of insert with 20 ng of vector (6:1).
insertBp / vectorBp = 0.33336 · 20 ng · (1000 / 3000) = 40 ngOpen this in the calculator to change the numbers.
Work out the result for ligation ratio.
Use 9 ng of insert with 15 ng of vector (3:1).
insertBp / vectorBp = 0.23 · 15 ng · (1000 / 5000) = 9 ngOpen this in the calculator to change the numbers.
More molecular biology practice
Resuspend a lyophilized primer to a stock concentration.
Convert a dsDNA mass to moles (and back) from its length.
DNA and RNA concentration and purity from absorbance.
Mass, molarity, and copies/µL for dsDNA, ssDNA, or ssRNA, both directions.
Open it in the calculator and it shows the same working step by step, with the units checked and the hazards flagged. Free, no account, and it keeps working offline once the page has loaded.
Open the Ligation ratio calculator