Molecular biology

Oligo resuspension: practice problems

Work out the result for oligo resuspension. Every answer here is computed by the same tested calculator the rest of the site uses, so a solution can never drift away from the maths.

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Problem 1 Oligo resuspension

Work out the result for oligo resuspension.

  • Amount (nmol): 40
  • Target stock (µM): 100
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Add 400 µL of buffer (TE or water) to make a 100 µM stock.

  1. Solve for volume: volume_µL = (nmol / targetConc_µM) · 1000
  2. Substitute: volume_µL = (40 nmol / 100 µM) · 1000 = 400 µL

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Problem 2 Oligo resuspension

Work out the result for oligo resuspension.

  • Amount (nmol): 32.5
  • Target stock (µM): 100
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Add 325 µL of buffer (TE or water) to make a 100 µM stock.

  1. Solve for volume: volume_µL = (nmol / targetConc_µM) · 1000
  2. Substitute: volume_µL = (32.5 nmol / 100 µM) · 1000 = 325 µL

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Problem 3 Oligo resuspension

Work out the result for oligo resuspension.

  • Amount (nmol): 40
  • Target stock (µM): 400
Show the answer and the working

Add 100 µL of buffer (TE or water) to make a 400 µM stock.

  1. Solve for volume: volume_µL = (nmol / targetConc_µM) · 1000
  2. Substitute: volume_µL = (40 nmol / 400 µM) · 1000 = 100 µL

Open this in the calculator to change the numbers.

Problem 4 Oligo resuspension

Work out the result for oligo resuspension.

  • Amount (nmol): 50
  • Target stock (µM): 400
Show the answer and the working

Add 125 µL of buffer (TE or water) to make a 400 µM stock.

  1. Solve for volume: volume_µL = (nmol / targetConc_µM) · 1000
  2. Substitute: volume_µL = (50 nmol / 400 µM) · 1000 = 125 µL

Open this in the calculator to change the numbers.

Problem 5 Oligo resuspension

Work out the result for oligo resuspension.

  • Amount (nmol): 30
  • Target stock (µM): 400
Show the answer and the working

Add 75 µL of buffer (TE or water) to make a 400 µM stock.

  1. Solve for volume: volume_µL = (nmol / targetConc_µM) · 1000
  2. Substitute: volume_µL = (30 nmol / 400 µM) · 1000 = 75 µL

Open this in the calculator to change the numbers.

Problem 6 Oligo resuspension

Work out the result for oligo resuspension.

  • Amount (nmol): 32.5
  • Target stock (µM): 750
Show the answer and the working

Add 43.33 µL of buffer (TE or water) to make a 750 µM stock.

  1. Solve for volume: volume_µL = (nmol / targetConc_µM) · 1000
  2. Substitute: volume_µL = (32.5 nmol / 750 µM) · 1000 = 43.33 µL

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Problem 7 Oligo resuspension

Work out the result for oligo resuspension.

  • Amount (nmol): 10
  • Target stock (µM): 250
Show the answer and the working

Add 40 µL of buffer (TE or water) to make a 250 µM stock.

  1. Solve for volume: volume_µL = (nmol / targetConc_µM) · 1000
  2. Substitute: volume_µL = (10 nmol / 250 µM) · 1000 = 40 µL

Open this in the calculator to change the numbers.

Problem 8 Oligo resuspension

Work out the result for oligo resuspension.

  • Amount (nmol): 32.5
  • Target stock (µM): 150
Show the answer and the working

Add 216.7 µL of buffer (TE or water) to make a 150 µM stock.

  1. Solve for volume: volume_µL = (nmol / targetConc_µM) · 1000
  2. Substitute: volume_µL = (32.5 nmol / 150 µM) · 1000 = 216.7 µL

Open this in the calculator to change the numbers.

Problem 9 Oligo resuspension

Work out the result for oligo resuspension.

  • Amount (nmol): 45
  • Target stock (µM): 100
Show the answer and the working

Add 450 µL of buffer (TE or water) to make a 100 µM stock.

  1. Solve for volume: volume_µL = (nmol / targetConc_µM) · 1000
  2. Substitute: volume_µL = (45 nmol / 100 µM) · 1000 = 450 µL

Open this in the calculator to change the numbers.

Problem 10 Oligo resuspension

Work out the result for oligo resuspension.

  • Amount (nmol): 15
  • Target stock (µM): 100
Show the answer and the working

Add 150 µL of buffer (TE or water) to make a 100 µM stock.

  1. Solve for volume: volume_µL = (nmol / targetConc_µM) · 1000
  2. Substitute: volume_µL = (15 nmol / 100 µM) · 1000 = 150 µL

Open this in the calculator to change the numbers.

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