SDS-PAGE gel: practice problems Work out the volume of every component. Every answer here is computed by the same tested calculator the rest of the site uses, so a solution can never drift away from the maths.
Problem 1 SDS-PAGE gel Work out the volume of every component.
Acrylamide %: 9.5 Gel volume: 11 mL Show the answer and the working 9.5% resolving gel, 11 mL total. Combine:
Acrylamide: V · (% / 30) = 11 · (9.5 / 30) = 3.483 mL Tris (1.5 M Tris-HCl pH 8.8): V · 0.25 = 11 · 0.25 = 2.75 mL SDS 10%: V · 0.01 = 0.11 mL APS 10%: V · 0.01 = 0.11 mL TEMED: V · 0.0004 = 0.0044 mL Water (by difference): V − (acrylamide + Tris + SDS + APS + TEMED) = 4.542 mL Open this in the calculator to change the numbers.
Problem 2 SDS-PAGE gel Work out the volume of every component.
Gel layer: Stacking Acrylamide %: 4 Gel volume: 4 mL Show the answer and the working 4% stacking gel, 4 mL total. Combine:
Acrylamide: V · (% / 30) = 4 · (4 / 30) = 0.5333 mL Tris (1.0 M Tris-HCl pH 6.8): V · 0.125 = 4 · 0.125 = 0.5 mL SDS 10%: V · 0.01 = 0.04 mL APS 10%: V · 0.01 = 0.04 mL TEMED: V · 0.001 = 0.004 mL Water (by difference): V − (acrylamide + Tris + SDS + APS + TEMED) = 2.883 mL Open this in the calculator to change the numbers.
Problem 3 SDS-PAGE gel Work out the volume of every component.
Acrylamide %: 8 Gel volume: 10 mL Show the answer and the working 8% resolving gel, 10 mL total. Combine:
Acrylamide: V · (% / 30) = 10 · (8 / 30) = 2.667 mL Tris (1.5 M Tris-HCl pH 8.8): V · 0.25 = 10 · 0.25 = 2.5 mL SDS 10%: V · 0.01 = 0.1 mL APS 10%: V · 0.01 = 0.1 mL TEMED: V · 0.0004 = 0.004 mL Water (by difference): V − (acrylamide + Tris + SDS + APS + TEMED) = 4.629 mL Open this in the calculator to change the numbers.
Problem 4 SDS-PAGE gel Work out the volume of every component.
Gel layer: Stacking Acrylamide %: 4.5 Gel volume: 5 mL Show the answer and the working 4.5% stacking gel, 5 mL total. Combine:
Acrylamide: V · (% / 30) = 5 · (4.5 / 30) = 0.75 mL Tris (1.0 M Tris-HCl pH 6.8): V · 0.125 = 5 · 0.125 = 0.625 mL SDS 10%: V · 0.01 = 0.05 mL APS 10%: V · 0.01 = 0.05 mL TEMED: V · 0.001 = 0.005 mL Water (by difference): V − (acrylamide + Tris + SDS + APS + TEMED) = 3.52 mL Open this in the calculator to change the numbers.
Problem 5 SDS-PAGE gel Work out the volume of every component.
Acrylamide %: 13 Gel volume: 10 mL Show the answer and the working 13% resolving gel, 10 mL total. Combine:
Acrylamide: V · (% / 30) = 10 · (13 / 30) = 4.333 mL Tris (1.5 M Tris-HCl pH 8.8): V · 0.25 = 10 · 0.25 = 2.5 mL SDS 10%: V · 0.01 = 0.1 mL APS 10%: V · 0.01 = 0.1 mL TEMED: V · 0.0004 = 0.004 mL Water (by difference): V − (acrylamide + Tris + SDS + APS + TEMED) = 2.963 mL Open this in the calculator to change the numbers.
Problem 6 SDS-PAGE gel Work out the volume of every component.
Gel layer: Stacking Acrylamide %: 4.5 Gel volume: 3 mL Show the answer and the working 4.5% stacking gel, 3 mL total. Combine:
Acrylamide: V · (% / 30) = 3 · (4.5 / 30) = 0.45 mL Tris (1.0 M Tris-HCl pH 6.8): V · 0.125 = 3 · 0.125 = 0.375 mL SDS 10%: V · 0.01 = 0.03 mL APS 10%: V · 0.01 = 0.03 mL TEMED: V · 0.001 = 0.003 mL Water (by difference): V − (acrylamide + Tris + SDS + APS + TEMED) = 2.112 mL Open this in the calculator to change the numbers.
Problem 7 SDS-PAGE gel Work out the volume of every component.
Acrylamide %: 10.5 Gel volume: 10 mL Show the answer and the working 10.5% resolving gel, 10 mL total. Combine:
Acrylamide: V · (% / 30) = 10 · (10.5 / 30) = 3.5 mL Tris (1.5 M Tris-HCl pH 8.8): V · 0.25 = 10 · 0.25 = 2.5 mL SDS 10%: V · 0.01 = 0.1 mL APS 10%: V · 0.01 = 0.1 mL TEMED: V · 0.0004 = 0.004 mL Water (by difference): V − (acrylamide + Tris + SDS + APS + TEMED) = 3.796 mL Open this in the calculator to change the numbers.
Problem 8 SDS-PAGE gel Work out the volume of every component.
Gel layer: Stacking Acrylamide %: 8 Gel volume: 5.5 mL Show the answer and the working 8% stacking gel, 5.5 mL total. Combine:
Acrylamide: V · (% / 30) = 5.5 · (8 / 30) = 1.467 mL Tris (1.0 M Tris-HCl pH 6.8): V · 0.125 = 5.5 · 0.125 = 0.6875 mL SDS 10%: V · 0.01 = 0.055 mL APS 10%: V · 0.01 = 0.055 mL TEMED: V · 0.001 = 0.0055 mL Water (by difference): V − (acrylamide + Tris + SDS + APS + TEMED) = 3.23 mL Open this in the calculator to change the numbers.
Problem 9 SDS-PAGE gel Work out the volume of every component.
Acrylamide %: 9 Gel volume: 10.5 mL Show the answer and the working 9% resolving gel, 10.5 mL total. Combine:
Acrylamide: V · (% / 30) = 10.5 · (9 / 30) = 3.15 mL Tris (1.5 M Tris-HCl pH 8.8): V · 0.25 = 10.5 · 0.25 = 2.625 mL SDS 10%: V · 0.01 = 0.105 mL APS 10%: V · 0.01 = 0.105 mL TEMED: V · 0.0004 = 0.0042 mL Water (by difference): V − (acrylamide + Tris + SDS + APS + TEMED) = 4.511 mL Open this in the calculator to change the numbers.
Problem 10 SDS-PAGE gel Work out the volume of every component.
Gel layer: Stacking Acrylamide %: 4 Gel volume: 5 mL Show the answer and the working 4% stacking gel, 5 mL total. Combine:
Acrylamide: V · (% / 30) = 5 · (4 / 30) = 0.6667 mL Tris (1.0 M Tris-HCl pH 6.8): V · 0.125 = 5 · 0.125 = 0.625 mL SDS 10%: V · 0.01 = 0.05 mL APS 10%: V · 0.01 = 0.05 mL TEMED: V · 0.001 = 0.005 mL Water (by difference): V − (acrylamide + Tris + SDS + APS + TEMED) = 3.603 mL Open this in the calculator to change the numbers.
More gels practice
Same topic, different calculation Grams of agarose for a % gel and casting volume.
Stuck on one of them? Open it in the calculator and it shows the same working step by step, with the units checked and the hazards flagged. Free, no account, and it keeps working offline once the page has loaded.
Open the SDS-PAGE gel calculator