Molecular biology

Oligo melting temperature (Tm)

Nearest-neighbor Tm with Na+ and Mg2+ salt corrections. Not a Wallace guess.

Tm = ΔH / (ΔS + R·ln(C_T/x)), salt-corrected
A/C/G/T/U, up to 200 nt. Below 14 nt falls back to the Wallace rule (flagged).
Chelates Mg2+; subtracted in the monovalent-equivalent.
Protocol result
⚗ Aliquot
Oligo melting temperature (Tm) · Tm = ΔH / (ΔS + R·ln(C_T/x)), salt-corrected · the lab bench, calculated
Oligo Tm✓ Tm = ΔH / (ΔS + R·ln(C_T/x)), salt-corrected
Calculated live

Tm = 55.2 °C.

1

GC content 50%, self-complementary.

Tm
55.2 °C
Method
nearest-neighbor
ΔH / ΔS
-161.2 kcal/mol, -438.4 cal/mol/K
GC
50%
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Oligo melting temperature (Tm), Aliquot