Protein

Standard curve

Fit a linear or quadratic standard curve (Bradford, BCA, ELISA) and read unknowns.

signal = slope x conc + intercept
ConcentrationSignal
Protocol result
⚗ Aliquot
Standard curve · signal = slope · conc + intercept · the lab bench, calculated
Standard curve✓ signal = slope · conc + intercept
Calculated live

Fit: signal = 0.722 x conc + 0.0325 (R2 = 0.9999).

1

Linear fit: slope 0.722, intercept 0.0325.

SignalConcentrationNote
0.550.7168in range
0.921.229in range
Slope
0.722
Intercept
0.0325
R2
0.9999
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How do you calculate standard curve?
A worked example with every step shown, and where it usually goes wrong.
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