What you are given
- Amount (nmol): 45
- Target stock (µM): 100
Formula: V = nmol / C
Molecular biology
How do you calculate oligo resuspension?
Resuspend a lyophilized primer to a stock concentration. The relationship used is V = nmol / C.
Worked example
Every number below is computed by the calculator, not typed in
The working
- Solve for volume:
volume_µL = (nmol / targetConc_µM) · 1000 - Substitute:
volume_µL = (45 nmol / 100 µM) · 1000=450 µL
Add 450 µL of buffer (TE or water) to make a 100 µM stock.
What each symbol means
- V: Resuspension buffer volume (µL)
- n: Delivered oligo amount (nmol)
- C: Target stock concentration (µM)
Source: Oligo resuspension (nmol to µM stock). A printable one-page version is on the formula sheet.
Try it yourself
Two problems, with the working revealed
Problem 1 Oligo resuspension
Work out the result for oligo resuspension.
- Amount (nmol): 10
- Target stock (µM): 400
Show the answer and the working
Add 25 µL of buffer (TE or water) to make a 400 µM stock.
- Solve for volume:
volume_µL = (nmol / targetConc_µM) · 1000 - Substitute:
volume_µL = (10 nmol / 400 µM) · 1000=25 µL
Open this in the calculator to change the numbers.
Problem 2 Oligo resuspension
Work out the result for oligo resuspension.
- Amount (nmol): 45
- Target stock (µM): 150
Show the answer and the working
Add 300 µL of buffer (TE or water) to make a 150 µM stock.
- Solve for volume:
volume_µL = (nmol / targetConc_µM) · 1000 - Substitute:
volume_µL = (45 nmol / 150 µM) · 1000=300 µL
Open this in the calculator to change the numbers.
Do it with your own numbers.
The Oligo resuspension calculator is free, shows every step, flags the hazards, and works offline once loaded. No account, no signup.
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