Gels
Agarose gel
Grams of agarose for a % gel and casting volume.
agarose (g) = % / 100 · V (mL)
Protocol result
⚗ Aliquot
Agarose gel · agarose (g) = (% / 100) · V (mL) · the lab bench, calculated
Agarose gel✓ agarose (g) = (% / 100) · V (mL)
Weigh 15 g of agarose into 300 mL of buffer.
1
Weigh 15 g of agarose into a flask.
2
Add 300 mL of 1× running buffer (TAE/TBE).
3
Microwave to dissolve, cool to ~55 °C, add stain, then pour.
⚠
Typical agarose gels are 0.5-3%.
Agarose
15 g
Buffer
300 mL
Percentage
5%