Spectrophotometry
Protein extinction coefficient (A280)
Sequence to ε280, MW, and E0.1%, the Pace way. Feeds the A280 calculator.
ε280 = nW·5500 + nY·1490 + nSS·125
One-letter codes (case and whitespace ignored). Non-standard letters are excluded with a note, never guessed.
Enter a one-letter amino-acid sequence, e.g. MKWVTFISLLFLFSSAYS.
Oxidized adds 125 M⁻¹cm⁻¹ per disulfide (Pace).
Protocol result
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Check your inputs
Enter a one-letter amino-acid sequence, e.g. MKWVTFISLLFLFSSAYS.
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