Statistics
IC50 / EC50 (4PL fit)
Fit a dose-response curve and read off the IC50/EC50, Hill slope, and R².
y = D + (A − D) / (1 + (x/C)^B)
Concentration of each point. Must be positive (use a log-dose series).
Measured response at each dose, in the same order. At least 4 points.
Protocol result
⚗ Aliquot
IC50 / EC50 (4PL fit) · y = D + (A − D) / (1 + (x/C)^B) · the lab bench, calculated
4PL fit✓ y = D + (A − D) / (1 + (x/C)^B)
IC50 / EC50 = 10.005 (R² 1)
1
Fitted 4PL: low 0.002, high 100.029, Hill slope 0.999, in 4 iterations.
IC50 / EC50
10.005
Hill slope
0.999
R²
1
Low / high
0.002 / 100.029