Statistics

Bradford / BCA protein assay

Fit a standard curve, subtract the blank, and back-calculate protein with dilution.

C = (A − blank − intercept) / slope
Known concentrations of your standards.
Measured absorbance for each standard.
Absorbance of each unknown to back-calculate.
Blank reading subtracted from every value.
Multiplies the back-calculated concentration.
Protocol result
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Bradford / BCA protein assay · C = (A − blank − intercept) / slope · the lab bench, calculated
Bradford✓ C = (A − blank − intercept) / slope
Calculated live

Sample 1 = 5 (R² 1)

1

Linear (Bradford) curve, blank 0 subtracted, dilution 1×.

#SignalNetConcFlag
10.550.555in range
R²
1
Max std CV
0%
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How do you calculate bradford / BCA protein assay?
A worked example with every step shown, and where it usually goes wrong.
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Bradford / BCA protein assay, Aliquot