Statistics
Protein fold-change (Western)
Loading-control-normalized densitometry: relative density and fold change vs control.
fold = (target/loading) / mean(control target/loading)
Target band density for each control-group lane.
Loading-control band (e.g. actin) for each control lane.
Target band density for each treated-group lane.
Loading-control band for each treated lane.
Protocol result
⚗ Aliquot
Protein fold-change (Western) · fold = (target/loading) / mean(control target/loading) · the lab bench, calculated
Fold-change✓ fold = (target/loading) / mean(control target/loading)
Treated = 2× ± 0.182 (SEM, n=2)
1
Each lane normalized to its loading control, then divided by the control group mean (1.1).
| Group | n | Mean fold | SD | SEM |
|---|---|---|---|---|
| control | 2 | 1 | 0.129 | 0.091 |
| treated | 2 | 2 | 0.257 | 0.182 |
control fold
1×
treated fold
2×