Statistics

Michaelis-Menten (Vmax, Km)

Fit enzyme kinetics to read Vmax, Km, and (with [E]t) kcat and kcat/Km.

v = Vmax · [S] / (Km + [S])
Substrate concentration of each point.
Initial reaction rate at each [S], same order. At least 3 points.
Total enzyme concentration. If given, kcat = Vmax/[E]t and kcat/Km are reported.
Protocol result
⚗ Aliquot
Michaelis-Menten (Vmax, Km) · v = Vmax · [S] / (Km + [S]) · the lab bench, calculated
Michaelis-Menten✓ v = Vmax · [S] / (Km + [S])
Calculated live

Vmax = 99.975, Km = 4.992 (R² 1)

1

Km is the [S] at half-maximal rate; Vmax is the saturating rate. Fit in 3 iterations.

Vmax
99.975
Km
4.992
R²
1
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How do you calculate michaelis-Menten (Vmax, Km)?
A worked example with every step shown, and where it usually goes wrong.
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Michaelis-Menten (Vmax, Km), Aliquot