Molecular biology
Oligo resuspension
Resuspend a lyophilized primer to a stock concentration.
V = nmol / C
Protocol result
⚗ Aliquot
Oligo resuspension · volume (µL) = amount (nmol) / targetConc (µM) · 1000 · the lab bench, calculated
Oligo resuspension✓ volume (µL) = amount (nmol) / targetConc (µM) · 1000
Add 300 µL of buffer (TE or water) to make a 150 µM stock.
1
Spin the tube briefly so the dried pellet settles at the bottom.
2
Add 300 µL of buffer, vortex, and let it dissolve for a few minutes.
Amount
45 nmol
Target
150 µM
Add buffer
300 µL