Protein

Protein by A280

Protein concentration from absorbance and extinction coefficient.

c (mg/mL) = A280 / (E0.1% x path)
Shown is BSA (0.667). Enter your protein value (e.g. from ProtParam).
Protocol result
⚗ Aliquot
Protein by A280 · conc (mg/mL) = A280 / (E0.1% · path) · the lab bench, calculated
Protein A280✓ conc (mg/mL) = A280 / (E0.1% · path)
Calculated live

Your protein is 0.1499 mg/mL.

1

Concentration: 0.1499 mg/mL.

A280
0.1
Concentration
0.1499 mg/mL
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How do you calculate protein concentration from A280?
A worked example with every step shown, and where it usually goes wrong.
Practice problems
Ten problems with worked solutions, every answer computed by this calculator.
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Protein by A280, Aliquot