Molecular biology

Transformation efficiency

Colonies, plated fraction, and DNA mass to CFU per µg.

CFU/µg = colonies · (total / plated) / (ng / 1000)
Protocol result
⚗ Aliquot
Transformation efficiency · CFU/µg = colonies · (total / plated) / (ng / 1000) · the lab bench, calculated
Transformation✓ CFU/µg = colonies · (total / plated) / (ng / 1000)
Calculated live

Transformation efficiency 2.000e+8 CFU/µg.

1

Back-calculated 2000 total CFU for 1.000e-5 µg.

Efficiency
2.000e+8 CFU/µg
Total CFU
2000
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Transformation efficiency, Aliquot