Molecular biology

Transformation efficiency: practice problems

Work out the result for transformation efficiency. Every answer here is computed by the same tested calculator the rest of the site uses, so a solution can never drift away from the maths.

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Problem 1 Transformation efficiency

Work out the result for transformation efficiency.

  • Colonies counted: 200
  • DNA transformed (ng): 0.02
  • Volume plated (µL): 500
  • Total recovery volume (µL): 1500
Show the answer and the working

Transformation efficiency 3.000e+7 CFU/µg.

  1. Recovery factor: total / plated = 3x
  2. Efficiency: colonies · recovery / µg DNA = 3.000e+7 CFU/µg

Open this in the calculator to change the numbers.

Problem 2 Transformation efficiency

Work out the result for transformation efficiency.

  • Colonies counted: 200
  • DNA transformed (ng): 0.01
  • Volume plated (µL): 500
  • Total recovery volume (µL): 2500
Show the answer and the working

Transformation efficiency 1.000e+8 CFU/µg.

  1. Recovery factor: total / plated = 5x
  2. Efficiency: colonies · recovery / µg DNA = 1.000e+8 CFU/µg

Open this in the calculator to change the numbers.

Problem 3 Transformation efficiency

Work out the result for transformation efficiency.

  • Colonies counted: 250
  • DNA transformed (ng): 0.075
  • Volume plated (µL): 400
  • Total recovery volume (µL): 1000
Show the answer and the working

Transformation efficiency 8.333e+6 CFU/µg.

  1. Recovery factor: total / plated = 2.5x
  2. Efficiency: colonies · recovery / µg DNA = 8.333e+6 CFU/µg

Open this in the calculator to change the numbers.

Problem 4 Transformation efficiency

Work out the result for transformation efficiency.

  • Colonies counted: 200
  • DNA transformed (ng): 0.05
  • Volume plated (µL): 150
  • Total recovery volume (µL): 2000
Show the answer and the working

Transformation efficiency 5.333e+7 CFU/µg.

  1. Recovery factor: total / plated = 13.33x
  2. Efficiency: colonies · recovery / µg DNA = 5.333e+7 CFU/µg

Open this in the calculator to change the numbers.

Problem 5 Transformation efficiency

Work out the result for transformation efficiency.

  • Colonies counted: 100
  • DNA transformed (ng): 0.075
  • Volume plated (µL): 300
  • Total recovery volume (µL): 1000
Show the answer and the working

Transformation efficiency 4.444e+6 CFU/µg.

  1. Recovery factor: total / plated = 3.33x
  2. Efficiency: colonies · recovery / µg DNA = 4.444e+6 CFU/µg

Open this in the calculator to change the numbers.

Problem 6 Transformation efficiency

Work out the result for transformation efficiency.

  • Colonies counted: 200
  • DNA transformed (ng): 0.05
  • Volume plated (µL): 100
  • Total recovery volume (µL): 1000
Show the answer and the working

Transformation efficiency 4.000e+7 CFU/µg.

  1. Recovery factor: total / plated = 10x
  2. Efficiency: colonies · recovery / µg DNA = 4.000e+7 CFU/µg

Open this in the calculator to change the numbers.

Problem 7 Transformation efficiency

Work out the result for transformation efficiency.

  • Colonies counted: 100
  • DNA transformed (ng): 0.075
  • Volume plated (µL): 750
  • Total recovery volume (µL): 1000
Show the answer and the working

Transformation efficiency 1.778e+6 CFU/µg.

  1. Recovery factor: total / plated = 1.33x
  2. Efficiency: colonies · recovery / µg DNA = 1.778e+6 CFU/µg

Open this in the calculator to change the numbers.

Problem 8 Transformation efficiency

Work out the result for transformation efficiency.

  • Colonies counted: 400
  • DNA transformed (ng): 0.04
  • Volume plated (µL): 100
  • Total recovery volume (µL): 1000
Show the answer and the working

Transformation efficiency 1.000e+8 CFU/µg.

  1. Recovery factor: total / plated = 10x
  2. Efficiency: colonies · recovery / µg DNA = 1.000e+8 CFU/µg

Open this in the calculator to change the numbers.

Problem 9 Transformation efficiency

Work out the result for transformation efficiency.

  • Colonies counted: 400
  • DNA transformed (ng): 0.02
  • Volume plated (µL): 100
  • Total recovery volume (µL): 1000
Show the answer and the working

Transformation efficiency 2.000e+8 CFU/µg.

  1. Recovery factor: total / plated = 10x
  2. Efficiency: colonies · recovery / µg DNA = 2.000e+8 CFU/µg

Open this in the calculator to change the numbers.

Problem 10 Transformation efficiency

Work out the result for transformation efficiency.

  • Colonies counted: 250
  • DNA transformed (ng): 0.05
  • Volume plated (µL): 100
  • Total recovery volume (µL): 1000
Show the answer and the working

Transformation efficiency 5.000e+7 CFU/µg.

  1. Recovery factor: total / plated = 10x
  2. Efficiency: colonies · recovery / µg DNA = 5.000e+7 CFU/µg

Open this in the calculator to change the numbers.

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