What you are given
- Standard conc: 0, 2, 4, 6, 8, 10
- Standard signal: 0.05, 0.25, 0.45, 0.65, 0.85, 1.05
- Sample signal: 0.55
Formula: C = (A − blank − intercept) / slope
Statistics
How do you calculate bradford / BCA protein assay?
Fit a standard curve, subtract the blank, and back-calculate protein with dilution. The relationship used is C = (A − blank − intercept) / slope.
Worked example
Every number below is computed by the calculator, not typed in
The working
- Model:
Bradford (linear)=linear - Blank subtracted:
net = signal − blank=0 - R²:
standard curve=1 - Dilution factor:
applied to unknowns=1×
Sample 1 = 5 (R² 1)
Do it with your own numbers.
The Bradford / BCA protein assay calculator is free, shows every step, flags the hazards, and works offline once loaded. No account, no signup.
Open the calculator