Buffers
Buffer builder
Build a multi-component buffer from stocks and solids.
per component: C1V1 = C2V2, or mass = C x V x MW
ComponentTargetUnitFromMWStockSu
Protocol result
⚗ Aliquot
Buffer builder · mass = C * V * MW (solids); V1 = C2 * V2 / C1 (stocks); diluent = V2 - sum(V1) · the lab bench, calculated
Buffer builder✓ mass = C * V * MW (solids); V1 = C2 * V2 / C1 (stocks); diluent = V2 - sum(V1)
To make 500 mL of buffer, combine:
1
Dissolve the solids in most of the diluent, add the stock volumes, then top up to 500 mL.
| Component | Amount |
|---|---|
| NaCl | 4.383 g |
| Tris | 25 mL |
| Diluent (top up) | 475 mL |
Final volume
500 mL
Components
2
Diluent
475 mL