Buffers
Buffer harmonizer (reverse solver)
Target pH and stocks in, an exact volume-closed recipe out.
split by 10^(pH − pKa), then V = n / C_stock
NameTarget (M)Stock (M)MW (solid)
Protocol result
⚗ Aliquot
Buffer harmonizer (reverse solver) · split by 10^(pH − pKa), then V = n / C_stock · the lab bench, calculated
Harmonizer✓ split by 10^(pH − pKa), then V = n / C_stock
Recipe for 1000 mL of Tris at pH 7.4.
1
Tris base stock: 8.98 mL.
2
Tris acid stock: 41.02 mL.
3
NaCl: 30 mL.
4
Water: 920 mL.
| Component | Amount |
|---|---|
| Tris base stock | 8.98 mL |
| Tris acid stock | 41.02 mL |
| NaCl | 30 mL |
| Water | 920 mL |