Buffers
Buffer pH temperature corrector
Dial in the bench pH so your buffer hits target at working temperature.
pH_bench = pH_target − (dpKa/dT)·(T_work − T_prep)
Blank uses the sourced value for the selected buffer.
Protocol result
⚗ Aliquot
Buffer pH temperature corrector · pH_bench = pH_target − (dpKa/dT)·(T_work − T_prep) · the lab bench, calculated
Buffer temp✓ pH_bench = pH_target − (dpKa/dT)·(T_work − T_prep)
Set the bench pH to 7.84 at 25 C to read 7.5 at 37 C.
1
Uncorrected, a buffer set to 7.5 at prep temp reads 7.16 at working temp.
Bench pH
7.84
pH drift
-0.34
pKa at working T
7.72