What you are given
- Substrate [S]: 0.5, 1, 2, 5, 10, 20, 50
- Rate (v): 9.1, 16.7, 28.6, 50, 66.7, 80, 90.9
Formula: v = Vmax · [S] / (Km + [S])
Statistics
How do you calculate michaelis-Menten (Vmax, Km)?
Fit enzyme kinetics to read Vmax, Km, and (with [E]t) kcat and kcat/Km. The relationship used is v = Vmax · [S] / (Km + [S]).
Worked example
Every number below is computed by the calculator, not typed in
The working
- Vmax:
asymptotic rate=99.975 - Km:
[S] at ½ Vmax=4.992 - R²:
1 − SS_res/SS_tot=1
Vmax = 99.975, Km = 4.992 (R² 1)
Do it with your own numbers.
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