What you are given
- Control target: 100, 120
- Control loading: 100, 100
- Treated target: 200, 240
- Treated loading: 100, 100
Formula: fold = (target/loading) / mean(control target/loading)
Statistics
How do you calculate protein fold-change (Western)?
Loading-control-normalized densitometry: relative density and fold change vs control. The relationship used is fold = (target/loading) / mean(control target/loading).
Worked example
Every number below is computed by the calculator, not typed in
The working
- Relative density:
target / loading control=per sample - Control mean:
mean of "control"=1.1 - Fold change:
rel. density / control mean=per sample
Treated = 2× ± 0.182 (SEM, n=2)
Do it with your own numbers.
The Protein fold-change (Western) calculator is free, shows every step, flags the hazards, and works offline once loaded. No account, no signup.
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