What you are given
- Buffer: Tris (pKa 8.06)
- Target pH: 7.4
- Buffer concentration (M): 0.05
- Final volume (mL): 1000
- Acid stock (M): 1
- Base stock (M): 1
Formula: split by 10^(pH − pKa), then V = n / C_stock
Buffers
How do you calculate buffer harmonizer (reverse solver)?
Target pH and stocks in, an exact volume-closed recipe out. The relationship used is split by 10^(pH − pKa), then V = n / C_stock.
Worked example
Every number below is computed by the calculator, not typed in
The working
- Ratio:
[A⁻]/[HA] = 10^(pH − pKa)=0.219 - Buffering moles:
C · V=50 mmol - Split:
base / acid=8.975 / 41.025 mmol
Recipe for 1000 mL of Tris at pH 7.4.
Do it with your own numbers.
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