What you are given
- Buffer: Tris (pKa 8.06)
- Target pH (at working temp): 7.5
- Working temperature (C): 37
- Prep / bench temperature (C): 25
Formula: pH_bench = pH_target − (dpKa/dT)·(T_work − T_prep)
Buffers
How do you calculate buffer pH temperature corrector?
Dial in the bench pH so your buffer hits target at working temperature. The relationship used is pH_bench = pH_target − (dpKa/dT)·(T_work − T_prep).
Worked example
Every number below is computed by the calculator, not typed in
The working
- ΔT:
T_work − T_prep=12 C - pH drift:
(dpKa/dT)·ΔT=-0.336 - Bench pH:
pH_target − drift=7.836
Set the bench pH to 7.84 at 25 C to read 7.5 at 37 C.
Try it yourself
Two problems, with the working revealed
Problem 1 Buffer pH temperature corrector
Work out the result for buffer pH temperature corrector.
- Buffer: Tris (pKa 8.06)
- Target pH (at working temp): 7
- Working temperature (C): 25
- Prep / bench temperature (C): 15
Show the answer and the working
Set the bench pH to 7.28 at 15 C to read 7 at 25 C.
- ΔT:
T_work − T_prep=10 C - pH drift:
(dpKa/dT)·ΔT=-0.28 - Bench pH:
pH_target − drift=7.28
Open this in the calculator to change the numbers.
Problem 2 Buffer pH temperature corrector
Work out the result for buffer pH temperature corrector.
- Buffer: Tris (pKa 8.06)
- Target pH (at working temp): 8
- Working temperature (C): 75
- Prep / bench temperature (C): 10
Show the answer and the working
Set the bench pH to 9.82 at 10 C to read 8 at 75 C.
- ΔT:
T_work − T_prep=65 C - pH drift:
(dpKa/dT)·ΔT=-1.82 - Bench pH:
pH_target − drift=9.82
Open this in the calculator to change the numbers.
Do it with your own numbers.
The Buffer pH temperature corrector calculator is free, shows every step, flags the hazards, and works offline once loaded. No account, no signup.
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